Over the past year we have been testing different variations of weed (flower, hash, wax, rosin etc), for cannabinoids concentration (THC, CBD, CBN), with what started as handheld machines and has now been upgraded to a gas chromatograph (GC-FID). What I can say is, a chromatograph is an instrument while these (not all) handheld machines seem to be devices, which aren't as accurate. There is a stage higher that is a Liquid Chromatograph (LC) which is more accurate, though we want to test for terpenes so GC is better suited. It would be great to share my findings with the great LB peeps. Typical readings were:
Just curious here , have you a qualified Science background ? . I have just finalised the license for a 6000m^2 cultivation facility located in Europe , for the production of high THC Medical cannabis. The place won't be finished until end of August/September though . Send a DM if interested.
Just background in physics, biology chemistry and biomedical science, but wouldn't say I'm qualified. Now that I have a live example of what to apply my knowledge to, I'm like 'what did that teacher say again?..!' It is still a learning curve and this seems the best place to share findings and data, for all the awesome Biggaz who are interested, feel free to DM any questions I'll share what I know :)
Thank you, it can test for an amount of a compound, provided it can be extracted via solvent and turned into a gas. Edibles have alot of compounds that aren't volatile, which can interfere with extracting and detecting the compounds of intersest. It would be easier to test what is going into the edibles and estimate per piece its potency, assuming it was mixed uniformly.
Flash chromatography, where you freeze the edible, pulverise, dissolve in solvent and put through a glass tube (column) with high pressure air, through a sieve of sorts (silica, sand etc) and this separates the cannabinoids from the sugars, fats etc in the edibles. The separated mixture can then be tested in a GC or LC, with much more accurate results.
Thank you! With chromatography alot of light can be shed on the quantity of a compound (quantification), and the identification of a compound (qualification).
With terpine profiles, which we are beginning to notice a more prominent uniqueness across different strains, the identification of strains might be possible based on chemical signatures alone, without the need of dna profiling.
The sample prep is a bit more complex for terpenes so will need to be a separate test.
In the uk there are only a few pesticides used, as mostly there are indoor grows. A chromatograph can be calibrated for any volatile compound, with a reference chemical standard, of known concentration. We acquire standards from sigma-Aldrich and Restek.
Resin is a term applied to the vast majority of concentrates from hash to shatter but rosin is specifically pressed with heat in order to extract without the use of solvents. Rosin is therefore a resin but resin is not necessarily rosin.
Resin needs expensive equipment to purge the residual solvent that's left from the solvent used in extraction, this is often not done perfectly. Rosin just uses pressure and heat. I've been pressing for some time now, it is fascinating to see the different textures and terpenes that come out it really is trial and error with temp and pressure, where you aren't destroying terpenes or darkening the finished product.